The Laboratory of 3D Functional Network and Dendritic Imaging is a multidisciplinary research team having laboratories at the IEM HAS Institute of Experimental Medicine of Hungarian Academy of Sciences - classified as a Center of Excellence by the European Commission and Pazmany Peter Catholic University (PPCU). We believe research should be supported with continuous technological advances, that is why we closely cooperate with innovative companies.
Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals GergelyGergely Szalay, Linda Judák,Gergely Katona, Katalin Ócsai, Gábor Juhász, Máté Veress, Zoltán Szadai, András Fehér, Tamás Tompa,Balázs Chiovini, Pál Maák and Balázs Rózsa Neuron (2016)
Understanding neural computation requires methods such as 3D acousto-optical (AO) scanning that can simultaneously read out neural activity on both the somatic and dendritic scales. AO point scanning can increase measurement speed and signal-to-noise ratio (SNR) by several orders of magnitude, but high optical resolution requires long point-to-point switching time, which limits imaging capability. Here we present a novel technology, 3D DRIFT AO scanning, which can extend each scanning point to small 3D lines, surfaces, or volume elements for flexible and fast imaging of complex structures simultaneously in multiple locations. Our method was demonstrated by fast 3D recording of over 150 dendritic spines with 3D lines, over 100 somata with squares and cubes, or multiple spiny dendritic segments with surface and volume elements, including in behaving animals. Finally, a 4-fold improvement in total excitation efficiency resulted in about 500 × 500 × 650 μm scanning volume with genetically encoded calcium indicators (GECIs).
The European Research Council (ERC) funds top researchers who engage in pioneering research, at the frontier of knowledge in their field.Congratulations to Balazs Rozsa Institute of Experimental Medicine of the Hungarian Academy of Sciences (Hungary) who received international scientific prize and award in 2015.
Project: Restoration of visual perception by artificial stimulation performed by 3D EAO microscopy (VISONby3DSTIM) Read all appearance in the hungarian media.
Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo, Balazs Rozsa, Gergely Szalay, Gergely Katona. In: Alon Korngreen (ed.) Advanced Patch-Clamp Analysis for Neuroscientists, Neuromethods, pp213-245
PhD Programmes Research Projects available!
For a description of each theme and a list of available projects, please visit the Career page.